FIGURE 8.

Ribozyme concentration dependence of intermolecular cleavage in vivo. (A) Intracellular concentrations of chimeric R228, substrate, and noncleavable substrate snoRNAs with a G₊₁A mutation in yeast grown in media with 2% glucose or with 2% sucrose and galactose concentrations ranging from 0% to 2%. (B) An Eadie-Hofstee plot of the chimeric R228 snoRNA concentration dependence of rates of chimeric substrate snoRNA cleavage in yeast nuclei gives a k_cleav value of 0.037 ± 0.004 min⁻¹ and a K_M^S value of 1.0 ± 0.2 μM.