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. 2004 May;10(5):863–879. doi: 10.1261/rna.5234204

TABLE 2.

Kinetic parameters for ribozyme cleavage of oligonucleotide substrates under different salt conditions^a

	10 mM MgCl₂			2 mM MgCl₂−150 mM NaCl
R•S	k_cleavage min⁻¹	K_M^S μM	k_cleavage/K_M^S μM⁻¹ min⁻¹	^kcleavage min⁻¹	K_M^S μM	k_cleavage/K_M^S μM⁻¹ min⁻¹	k_off^{S^b} min⁻¹
R2•S23	1 ± 0.2	1.2 ± 0.3	0.9 ± 0.2	0.11 ± 0.01	11 ± 5.0	0.011 ± 0.004	>1
R2A₁₄G•S23U₋₅₅C ^G	0.63 ± 0.07	0.9 ± 0.1	0.73 ± 0.02	0.18 ± 0.01	15 ± 2.0	0.012 ± 0.002	>2
R2A₁₄G•S23U₋₅₅C ^G	0.51 ± 0.05	0.9 ± 0.2	0.47 ± 0.03	0.19 ± 0.03	8.2 ± 0.7	0.022 ± 0.005	>2
R2•S24	0.78 ± 0.04	0.4 ± 0.1	1.6 ± 0.2	0.31 ± 0.01	0.83 ± 0.02	0.38 ± 0.03	0.5 ± 0.2
R2A₁₄G•S24U₋₅₅C ^G	0.79 ± 0.02	0.68 ± 0.06	1.2 ± 0.15	0.24 ± 0.02	0.63 ± 0.1	0.40 ± 0.06	—
R2A₁₄G•S24U₋₅₅C ^G	0.5 ± 0.1	0.6 ± 0.2	0.8 ± 0.3	0.25 ± 0.02	0.77 ± 0.02	0.39 ± 0.02	0.11 ± 0.05
R2•S26	0.76 ± 0.06	0.53 ± 0.07	1.5 ± 0.3	0.26 ± 0.02	0.43 ± 0.02	0.6 ± 0.2	<0.1
R2A₁₄G•S26U₋₅₅C ^G	0.68 ± 0.07	0.61 ± 0.09	1.1 ± 0.06	0.26 ± 0.01	0.49 ± 0.06	0.5 ± 0.1	<0.1
R2A₁₄G•S26U₋₅₅C ^G	0.47 ± 0.05	0.70 ± 0.03	0.8 ± 0.4	0.25 ± 0.02	0.53 ± 0.22	0.6 ± 0.3	—
R2•S28	0.76 ± 0.01	0.6 ± 0.1	1.5 ± 0.3	0.27 ± 0.02	0.33 ± 0.03	0.8 ± 0.1	—

^aMean and range of values obtained from two experiments.

^bMeasured using pulse chase experiments as described previously (Hegg and Fedor 1995).