TABLE 3.
Oligonucleotides used for plasmid constructions
| Plasmids | Sense | Antisense | Primers |
| pGEM4-U32 | 5′-CCGGTAAAGCTTTCTCACAATATACC-3′ | 5′-GTCCTTGAAGCTTCTCGATATAATGAC-3′ | U3RNAF/URRNAR |
| PGEM4-U3D2 | 5′-CTGTGTCGACGTACTTCATCGATGAATCCAACTTGGTTGATGAG-3′ | 5′-CTCATCAACCAAGTTGGATTCATCGATGAAGTACGTCGACACTG-3′ | PUF/PUR |
| pGULR26 | 5′-GCACGGATCGATACTATCACACGTCGCAAGGTGAGAAGCCAACC-3′ | 5′-GATCGCGGCGCCATAGTCTGGAGCGAAGGTGGACTGCCATTAG-3′ | RHGLR/NLR26 |
| pGULR23 | 5′-CTAATGGCAGTCGACGAACGCTCCAGACTATGG-3′ | 5′-CCATAGTCTGGAGCGTTCGTCGACTGCCATTAG-3′ | PGULR23F/PGULR23R |
| pGULR220 | 5′-GGCAGTCGACCTTGCGACGTGTGTAAGGCGATGAATCCAAC-3′ | 5′-GTTGGATTCATCGCCTTACACACGTCGCAAGGTCGACTGCC-3′ | PGULR420F/PGULR420R |
| pGUR228 | 5′-GGTATATTACCTGGTAC/CGATGAATCCAACTTGG-3′ | 5′-CCAAGTTGGATTCATCGGTACCAGGTAATATACC-3′ | PGUR2F/PGUR2R |
| pGUR23 | 5′-CGTACTTCATCGATAGATAGTGTGCAGCGTTCGTGAGAAGCCAACC-3′ | 5′-GGTTGGCTTCTCACGAACGCTGCACACTATCTATCGATGAAGTACG-3′ | PGUR3F/PGUR3R |
| pGUR24 | 5′-GTGTGCAGCGTTGGTGAGAAGCC-3′ | 5′-GGCTTCTCACCAACGCTGCACAC-3′ | PGUR24F/PGUR24R |
| pGUR26 | 5′CGTACTTCATCGATAGATAGTGTGCAGCGAAGGTGAGAAGCCAACC-3′ | 5′GGTTGGCTTCTCACCTTCGCTGCACACTATCTATCGATGAAGTACG-3′ | PGUR6F/PGUR6R |
| pGULR23A | 5′-GTACTCTAATGGCAATCCACGAACGCTCC-3′ | 5′-GGAGCGTTCGTGGATTGCCATTAGAGTAC-3′ | PGULR23AF/PGULR23AR |
| pGULR26A | 5′-GTACTCTAATGGCAATCCACCTTCGCTCC-3′ | 5′-GGAGCGAAGGTGGATTGCCATTAGAGTAC-3′ | PLR26AF/PLR26AR |
| pGULR220A | 5′-GGTACTCTAATGGCAATCCACCTTGCGACG-3′ | 5′-CGTCGCAAGGTGGATTGCCATTAGAGTACC-3′ | PGULR220AF/PGULR220AR |
| pGUS | 5′-CGAACCACCTGGCAGTCCACCTTGCGACGTGTGATAGAT-3′ | 5′-CTATCACACGTCGCAAGGTGGACTGCCAGGTGGTT-3′ | 156/256 |
| pGUSA | 5′-GAACCACCTGGCAATCCACCTTGCGACG-3′ | 5′-CGTCGCAAGGTGGATTGCCAGGTGGTTC-3′ | SGA1/SGA2 |
| pGUSU-5 C | 5′-CATCGAACCACCCGGCAGTCCACCTTGC-3′ | 5′-GCAAGGTGGACTGCCGGGTGGTTCGATG-3′ | PGUSU5CF/PGUSU5CR |
| pGUSU-5 C | 5′-CATCGAACCACCGGGCAGTCCACCTTGC-3′ | 5′-GCAAGGTGGACTGCCCGGTGGTTCGATG-3′ | PGUSU5GF/PGUSU5GR |
| pGUR2A-14 C | 5′-CAAGGTGAGAAGCCCACCAGAGAAACAC-3′ | 5′-GTGTTTCTCTGGTGGGCTTCTCACCTTG-3′ | A14CF/A14CR |
| pGUR2A-14 C | 5′-CAAGGTGAGAAGCCGACCAGAGAAACAC-3′ | 5′-GTGTTTCTCTGGTCGGCTTCTCACCTTG-3′ | A14GF/A14GR |
| pU14 | 5′-GATCGCAGTTTCCACGGTAGGAGTACGCTTACGAACCCATCGTTAGTACTCTCGGTGACC-3′ | 5′-AGCTGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCGTACTCCTACCGTGGAAACTGC-3′ | U14F/U14R |