FIGURE 1.

Secondary structure of RNA molecules to which ribosomal protein S8 binds. (A,B) Helix 21 of 16S rRNA from T. thermophilus and E. coli, respectively. Yellow regions indicate S8 contacts within the T. thermophilus 30S (Brodersen et al. 2002). (C) Wild-type regulatory site in the spc operon mRNA from E. coli. The start codon for the L5 gene is shown in red, and the Shine-Dalgarno region is boxed. (D) mRNA constructs used in this study. Lowercase nucleotides were added for stability or transcriptional purposes and are not part of the wild-type sequence. The boxed construct was crystallized in complex with E. coli S8.