TABLE 2.

Site-specific integration efficiency of wtAAV and rAAV plasmid vectors^a

Plasmid	Total no. of clones screened	No. of clones with transgene	Integration efficiency (%)	% of transgene- containing clones with AAVS1 disruption
pSub201	269	33	12	90
pSub201 + Ad	114	10	9	90
pTRUF2	95	0	<1
p2ITRLacZ	48	0	<2
pRepGFP	166	22	13	<100
pGFPCap	194	10	5	100

^aHeLa cells were transfected with plasmid vectors, and clonal cell lines were grown for 6 weeks. Whole-cell DNA was harvested, digested with EcoRI, and separated on 1% agarose gels. DNA was transferred to nylon membranes and hybridized to ³²P-labeled transgene probes.