FIGURE 2.

Example of a RACE-PCR result. An ethidium-bromide-stained agarose gel giving an example of a cTnI RACE-PCR result. Antisense-specific RACE was done with primer 631F for 5′RACE and with primer 363R for 3′RACE. As a control, an intern fragment was amplified using primer 255F and 419R. The control PCR resulted in the expected fragment at 164 bp with the 5′ and 3′ RACE-ready cDNA as template, whereas the RACE-PCR produced only some faint bands (arrows) and a smear in the background. Because optimization was not effective, we cloned the complete RACE-PCR.