FIG. 8.

ZL₃VS and leupeptin differentially affect the subcellular distribution of MHC class I HC in K3-BJAB cells. (A) Quantitation of total MHC class I HC recovered in a W6/32 immunoprecipitation from the 10% Percoll gradient fractions from BJAB (squares) or K3-BJAB (circles) cells pulsed and chased in the absence (open symbols) or presence (closed symbols) of 50 μM ZL₃VS. Levels of MHC class I HC are plotted for each fraction as the percent area under the curve within each gradient. (B) Quantitation of total MHC class I HC at the top and bottom of a 10% Percoll gradient of BJAB and K3-BJAB cells after a 30-min pulse and 2-h chase in the presence of the inhibitors indicated.