FIGURE 2.

Editing of gld-2 mRNA is efficient and germline-specific. Reverse transcriptase reactions were performed with the same primer (indicated as a black arrow) on poly(A+) RNA from wild-type mixed-stage and q224 worms. q224 mutants possess essentially no germline (Kodoyianni et al. 1992). PCR products of 1464 and 1338 bp were obtained from the germline-specific mRNAs, corresponding to the mRNAs designated 4.7L and 4.7S in Figure 1B. A PCR product of 1269 bp was obtained from the soma-enriched transcripts, corresponding to the mRNA designated 4.6 in Figure 1B. The PCR products corresponding to the germline mRNAs are digested completely by AlwNI, demonstrating that they have been edited. In contrast, most (if not all) of the 1269-bp product is not cleaved, and so is not edited.