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Chromatography and purification of LeishIF4E-1 on m7GTP-sepharose. Crude extracts from noninduced BL21 bacterial cells (crude) and the soluble fraction (Sup) following induction by IPTG were applied onto a column of m7GTP-Sepharose. The flow-through (FT) was collected and the column was washed (Wash) first with equilibration buffer and then with equilibration buffer containing GTP (100 μM). The protein was eluted by a wash containing m7GTP (200 μM).
