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. 2004 Sep;10(9):1469–1478. doi: 10.1261/rna.7510504

FIGURE 6.

FIGURE 6.

Fluorescence titration measurements on binding of LeishIF4E-1 to cap analogs. LeishIF4E-1 proteins, at a concentration of 0.3 μM, were titrated by the cap analogs: cap-4 (open squares), m7GTP (open circles), m7GpppA (black squares), m7 Gpppm36,6,2′A (open triangles), and GTP (black triangles), at 20°C in 50 mM HEPES/NaOH, 100 mM NaCl, 1 mM EDTA, 1 mM DTT. Protein fluorescence, presented as relative values, was excited at 295 nm and observed at 345 nm. The observed increasing fluorescence intensity at a higher concentration of ligand originates from the emission of the free cap analogs in solution.