FIGURE 3.

Dcp1/Dcp2 activity in Ascaris translation extracts. Cap-labeled RNAs were incubated in Ascaris translation extracts with and without 200 μM m⁷GpppG, the reactions phenol:chloroform extracted, resolved by 25% denaturing PAGE, and products detected by autoradiography. Note the relative ratios of monophosphate (3′ to 5′ decay and scavenger activity) and diphosphate (Dcp1/Dcp2-like activity) nucleotide decay products in lanes 2 and 4. The diphosphate products do not increase dramatically when scavenger activity is inhibited with m⁷GpppG. Dcp1/Dcp2 products (m⁷Gp*p and m₃^2,2,7Gp*p), although limited, are highly reproducible.