FIGURE 6.

Comparison of cap substrate hydrolysis for recombinant C. elegans and human DcpS. Cap cleavage reactions were carried out at 30°C with recombinant C. elegans DcpS (A) or 37°C with recombinant human DcpS (B) for 30 min using TLC purified and characterized substrates. Reactions were carried out and analyzed as described in Figure 2C ▶ using 25% denaturing PAGE and detected by autoradiography. Control reactions using a similarly purified recombinant protein (β-galactosidase) demonstrated no degradation of RNA or cleavage of cap dinucleotides.