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. 2004 Oct;10(10):1625–1636. doi: 10.1261/rna.7270204

FIGURE 5.

FIGURE 5.

Both UGUA regions are required for in vivo regulation of COX17 mRNA deadenylation and decay by Puf3p. Shown are Northern blot analyses of transcriptional pulse-chase experiments examining decay of the COX17 transcript from wild-type PUF3 (A); wild-type PUF3, Site A mutant (B); wild-type PUF3, Site B mutant (C); puf3Δ (D), and wild-type PUF3, Site A + B mutant (E) strains. Minutes after transcriptional repression are indicated above each blot. The -8 lane in each blot corresponds to background levels of RNA expression prior to galactose induction of the COX17 transcript. The distribution of poly(A) tail lengths are indicated by arrows on the left of each blot, from a maximum length of 60 A’s (top) to a fully deadenylated length of 0 A’s (bottom).