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The COX17 3′ UTR is sufficient to promote Puf3p regulation of mRNA decay. Shown are Northern blot analyses of the decay of MFA2 mRNA or the hybrid MFA2/COX17 mRNA expressed from a wild-type (WT) strain or a puf3Δ strain. Minutes following transcriptional repression are indicated above the set of blots, with the half-lives (t1/2) as determined from multiple experiments.
