FIGURE 5.

HPLC chromatograms monitored at 290 nm. The positions of cmo⁵U, ho⁵U, and mo⁵U are indicated above the corresponding peaks. The three major nucleosides C, U, and G are indicated below the corresponding peaks. “x” indicates an unidentified compound with a spectrum that is not similar to ho⁵U, comigrating with ho⁵U in all analyses. About 25 μg of total tRNA was digested to nucleosides and analyzed. The degradation procedure used, the treatment by alkaline phosphatase at pH 8.3, hydrolyzes most of mcmo⁵U to cmo⁵U.