TABLE 1.

Quantification of cmo⁵U, ho⁵U, and mo⁵U compared to Ψ in tRNA from different strains

Strain	Relevant genotype	Growth conditions	ho5U/Ψa	mo5U/Ψ	cmo5U/Ψ
LT2	Wild type	100 klett	0	N/Db	0.082
LT2	Wild type	24 h	0	N/D	0.079
GT6854	cmoA1<>frt	100 klett	0.041	0.036	N/D
		24 h	0.0017	0.087	N/D
GT6945	cmoA4<>frt (ΔSAM)	100 klett	0.084	N/D	N/D
GT6856	cmoB2<>frt	100 klett	0.072	N/D	N/D
GT6858	cmoAB3<>frt	100 klett	0.072	N/D	N/D
GT6909	aroD553::Tn10	100 klett	0.014	0.0081	N/D
		24 h	0.011	0.023	N/D

The area of each compound monitored at 290 nm was divided with that of pseudouridine (Ψ) at 254 nm. Cells were either harvested at 100 klett units (about 4 × 10⁸ cells/mL) or after incubation of the culture for 24 h.

^aAn unknown compound, comigrating with ho⁵U, was present in all samples, so all values for ho⁵U are corrected by subtracting the value found in tRNA from strain LT2 at the same conditions.

^b(N/D) Not detected.