TABLE 1.
Bacterial strains, plasmids and oligodeoxynucleotide primers
| Description | Reference | |
| Strains | ||
| Acinetobacter calcoaceticus ADP1 | Wild type | Juni 1972 |
| A. calcoaceticus ASD89 | Derived from ADP1: rlmAI, KanR | This study |
| Escherichia coli | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80d lacZΔM15 ΔlacX74 | Life |
| DH10B | deoR recA1 endA1 araD139 Δ (ara, leu)7697 galU galK λrpsL nupG | Technologies, Inc. |
| E. coli IB10 | thr leu his argA relA rrmA10 | Björk and Isaksson 1970 |
| E. coli TA531 | Δ (rrnE rrnB rrnH rrnA) rrnG::LacZ+rrnD::cat+rrnC::cat+ ΔrecA/pTRNA66, pHK-rrnC+ | Asai et al. 1999 |
| Plasmids | ||
| pHK-rrnC+ | Contains the entire, wild-type E. coli rrnC; used to maintain E. coli strain TA531: KanR | Asai et al. 1999 |
| pNK | Contains the entire, wild-type E. coli rrnB: AmpR | Xiong et al. 2000 |
| pSD50 | Acinetobacter rlmA fragment cloned in pUC19 at HincII: AmpR | This study |
| pSD82 | Acinetobacter HindIII genome fragment with complete rlmAI sequence; cloned in pUC19: AmpR | This study |
| pSD88 | Acinetobacter rlmAI gene cloned in pUHE24-2 at NedI/BglII: AmpR | This study |
| pSD88-KanR | Disruption of pSD88 rlmAI gene at Eco47III: AmpR, KanR | This study |
| pSD114 | Streptomyces fradiae rlmAII (tlrB) gene cloned in pWH845: AmpR, laclq | This study |
| pSD118 | Corynebacterium glutamicum pAG1 rlmAII gene cloned in pWH845: AmpR, laclq | This study |
| pSD189 | Acinetobacter rlmAI gene cloned in pWH845: AmpR, laclq | This study |
| pUC19 | Cloning/sequencing vector: AmpR | Yanisch-Perron et al. 1985 |
| pUHE24-2 | Cloning/expression vector: AmpR | Bujard et al. 1987 |
| pWH845 | Acinetobacter/E.coli shuttle vector: AmpR, laclq | Schirmer et al. 1997 |
AmpR and KanR indicate ampicillin and kanamycin resistance.