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Effect of disruption of the base triple on the reaction equilibrium
| (kcat/Km)G | (kcat/Km)GA | (kcat/Km)G | |
| Ribozyme | (M−1 min−1) | ((kcat/Km)GA | |
| Wild type | 280 ± 60 | 1.9 ± 0.1 | 150 ± 24 |
| C305 | 50 ± 14 | 1.5 ± 0.1 | 33 ± 7 |
| (C111·G209) | 10 ± 5 | 3.0 ± 0.4 | 33 ± 1.6 |
| (C111 · G209) · C305) | 1820 ± 300 | 3.0 ± 1.5 | 610 ± 80 |
All experiments were performed at 30°C, pH 7.2. −1d,rSA and −1d,rP were used to ensure that the chemical step was rate-limiting in all cases.
