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LeishIF4E-1 is encoded by a single-copy gene. Genomic DNA was digested with different restriction enzymes and separated over a 0.8% agarose gel. The blot was hybridized with a probe derived from the complete coding region of LeishIF4E-1 (645 bp). A map describing the genomic locus is shown below the blot. (E) EcoRI; (M) MspI; (N) NruI; (S) SacII; (X) XhoI; (nd) nondigested.
