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. 2004 Nov;10(11):1783–1797. doi: 10.1261/rna.7154104

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FIGURE 6. — rpr and Dm-hsp70 5′UTR IRESs are functional during heat shock. Monocistronic mRNA reporter expression in translation extracts derived from untreated (A) and heat-shocked (B) embryos. Normalized activity of capped (C) and ApppG-capped (D) dicistronic mRNA reporters in translation extracts derived from heat-shocked embryos. In all experiments, the normalization is referred to the hsp70-containing reporter mRNA. (E) Absolute values of reporter gene expression activity of 7mGpppG-capped transcripts in heat shock extracts. Cap-dependent translation is abolished. (F) Absolute values of reporter gene expression activity of ApppG-capped transcripts in heat shock extracts.