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Pre-rRNA processing in Saccharomyces cerevisiae. (A) Structure of the rDNA transcription unit, including the various processing sites. (B) The pre-rRNA processing pathway. The processing steps in ITS1 involving RNase MRP and the 5′ → 3′ exonucleases Xrn1p and Rat1p are indicated. (C) Enlargement of the central portion of the pre-rRNA showing the positions of the different probes used for Northern and primer extension analysis. See Materials and Methods for the sequences of the probes.
