FIGURE 6.

Effect of inactivation of the RNT1 gene on growth and pre-rRNA processing in cells dependent upon the Rrp5-Δ3p mutant protein. Strain YJV204 [Gal::rrp5/RNT1/p(rrp5-Δ3)] and FVY07A [Gal::rrp5/rnt1::KAN/p(rrp5-Δ3)] were grown on galactose-based medium to midexponential phase and plated in 10-fold serial dilutions on either galactose or glucose plates (A). The same strains were grown on galactose-based medium and shifted to glucose-based medium. Samples were taken immediately before and 8 h after the shift, either directly (- LiCl) or after treatment of the culture with 0.2 M LiCl for 1 h (+ LiCl). Total RNA was isolated and subjected to Northern analysis using probe 4 (B) or probe 2 (C) or primer extension analysis using probe 4 (D).