FIGURE 3.

Translational elongation mediated by Hygromycin does not protect transcripts from RNAi mediated degradation. (A) Three hours after U1_GFP was transfected into HEK293 cells, Hygromycin (10 nM final concentration) was added to the medium with or without siRNA against EGFP. EGFP expression was monitored 21 h later. (B) EGFP mRNA levels were analyzed by RT-PCR as described in the legend to Figure 2.