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. 2005 Feb;11(2):128–138. doi: 10.1261/rna.7114505

TABLE 1.

Sets of adjacent Arabidopsis loci expressing elongated transcripts

Two ORFs One ORF
RIKEN clone Category Locus RIKEN clone Category Locus
RAFL05-11-B17* A At4g00030/At4g00040 RAFL05-02-O09 P At1g33470/At1g33475
RAFL09-22-H13* A At2g25610/At2g25620 RAFL06-69-H17 P At2g35710/At2g35715
RAFL09-40-B19 A At3g06510/At3g06520 RAFL07-15-G11* P At5g35980/At5g35990
RAFL09-54-M24 A At2g24940/At2g24945 RAFL09-16-G02 P At1g23430/At1g23440
RAFL09-63-M16* A At2g25610/At2g25620 RAFL09-44-D01 P At4g29490/At4g29500
RAFL11-04-B08 A At4g34530/At4g34540 RAFL09-60-B14 P At1g60200/At1g60210
RAFL16-76-N11 A At5g53480/At5g53485 RAFL09-61-C04 P At4g31080/At4g31090
RAFL09-60-E22 B At3g45640/At3g45650 RAFL09-81-M20* P At5g54120/At5g54130
RAFL19-62-F22 B At3g48565/At3g48570 RAFL15-41-F10 P At4g20120/At4g20130
RAFL08-16-I18 C At3g26420/At3g26430 RAFL16-67-A17 P At5g20530/At5g20540
RAFL09-13-P07 D At3g53130/At3g53140 RAFL16-69-P16 P At5g65350/At5g65360
RAFL08-09-I21* E At1g23870/At1g23880 RAFL19-84-B16 P At4g33330/At4g33340
RAFL21-32-H16* F At4g29850/At4g29860 RAFL05-11-G13 Q At2g39070/At2g39080
RAFL16-98-M19* G At3g13700/At3g13710 RAFL06-74-D12 Q At1g26280/At1g26300
RAFL09-29-J06 H At5g57140/At5g57150 RAFL07-15-F13* Q At2g40835/At2g40840
RAFL09-67-C10 I At4g10955/At4g10960 RAFL09-24-E16* Q At1g09980/At1g09990
RAFL09-69-N10 J At3g20080/At3g20083 RAFL14-80-M23 Q At1g62850/At1g62855
RAFL14-83-D16 K At5g50970/At5g50980 RAFL16-01-C03 Q At5g63420/At5g63430
RAFL16-97-D24* K At3g59560/At3g59570 RAFL09-56-O16 R At5g55030/At5g55040
RAFL08-13-H11* L At3g20080/At3g20083 RAFL05-09-I21 S At5g48250/At5g48260
RAFL09-72-F15* M At2g31330/At2g31340 RAFL06-10-K24* S At2g03390/At2g03400
RAFL15-09-P04* N At3g03350/At3g03360 RAFL09-32-C08* S At5g04300/At5g04310
RAFL05-21-L13 O At4g35870/At4g35880 RAFL15-09-A22* S At2g31110/At2g31120
RAFL07-17-D11* O At3g26300/At3g26310 RAFL16-73-D01* S At1g31700/At1g31710
RAFL08-11-J20* O At1g01770/At1g01780 RAFL09-85-I15* T At3g48900/At3g48910
RAFL08-19-A05 O At4g20830/At4g20840 RAFL11-13-C20 U At5g12160/At5g12170
RAFL09-34-K18 O At1g11350/At1g11360 RAFL08-08-P22 V At3g16855/At3g16857
RAFL16-96-P09 O At1g73060/At1g73070 RAFL08-17-N09* V At3g52640/At3g52650
RAFL19-66-K06 O At5g23230/At5g23240 RAFL21-17-J10 V At3g08830/At3g08840
RAFL09-21-B11* W At3g24460/At3g24470
RAFL07-12-J11 W At5g62590/At5g62600

Asterisks designate transcripts containing additional ATG codons in the 5′ UTR of the first ORF that potentially initiate on internal ATG codons. Bold type designates adjacent loci whose ability to generate bicistronic transcripts or fused monocistronic transcripts has been confirmed by RT-PCR gel blot analysis of Arabidopsis RNAs shown in Figure 7. Transcripts derived from enviromentally stressed plants include five and seven clones in the RAFL5 and RAFL8 libraries constructed with plants treated with dehydration, 3 and 21 clones in the RAFL6 and RAFL9 libraries constructed with plants at different developmental stages as well as plants treated with dehydration and cold, four in the RAFL7 library constructed with plants treated with cold, two in the RAFL11 library constructed with plants at different developmental stages as well as plants treated with dehydration, cold, salt, heat, UV, or ABA, and two in the RAFL21 library constructed with plants treated with environmental stresses (heat, UV) and various chemical treatments (ABA, auxin, ethylene, JA, SA, GA, cytokinin, BTH) (Seki et al. 2002). Transcripts derived from unstressed plants include two in the RAFL14 library constructed with root tissue, three and three in the RAFL15 and RAFL19 libraries constructed with silique and flower tissues, and eight in the RAFL16 library constructed with dark-grown plants (Seki et al. 2002).