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Single-turnover kinetic studies of the MSL1L2 HHRz. (A) Rate curves of MSL1L2 in 0.1 (•), 1 (♦), and 10 (▪) mM Mg2+ in 5 mM HEPES (pH 7.0) and 100 mM Na+ fit to a biphasic rate equation (Equation 2). (Inset) Expansion from 0- to 5-min time points. (B) kobs for fast (•) and slow (♦) populations as a function of Mg2+ concentration.
