FIGURE 8.

Single-turnover kinetic study of the MSL1L2 U Loop HHRz. Kinetic traces of WT MSL1L2 (•) and U Loop (▪) in 5 mM HEPES (pH 7.0), 100 mM Na⁺, and 10 mM Mg²⁺. WT MSL1L2 data are fit to a biphasic equation (Equation 2) with a fast k_obs of >6 min⁻¹ and a slow k_obs of 0.14 min⁻¹. Data for U Loop MSL1L2 are fit to a monophasic rate equation (Equation 1), and a k_obs of 0.02 min⁻¹ is obtained.