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Analytical ultracentrifugation of the PSIV IRES. (A) Measured sedimentation coefficient, normalized to standard conditions, as a function of [RNA]. Values at 0 and 20 mM MgCl2 are shown, and the extrapolation to 0 [RNA] is depicted as a dotted line. (B) Measured sedimentation coefficient as a function of [MgCl2] for the PSIV IRES.
