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Experimental design. (A) In the absence of rapamycin*, the FRB*-RS fusion protein is not recruited to the RNA. As a result, splicing of the intron does not occur. (B) In the presence of rapamycin*, the FRB*-RS fusion protein is recruited to the RNA via the MS2-F3 protein and splicing of the intron occurs. (C) The structure of rapamycin*. (D) The structure of FK506. The surface of each molecule that interacts with FKBP is indicated.
