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Synthesis of antisense and sense RNA in P. falciparum nuclei from synchronous parasites. Nascent RNA in synchronous, schizont-stage erythrocytic parasite nuclei was labeled with [α-32P]UTP and hybridized to filters containing single-stranded DNA. Filters were washed, treated with 10 μg/mL RNase A, and analyzed by PhosphorImaging. The DNA targets were the same as described in the Figure 2 legend.
