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. 2005 Apr;11(4):447–458. doi: 10.1261/rna.7255805

FIGURE 5.

FIGURE 5.

Mutational analysis of the core binding element. (A) Double nucleotide substitutions in the FBEa. Substitutions are indicated in lowercase; core element is boxed. β-Galactosidase values are relative to the wild-type FBEa; error bars represent the standard deviation of six repetitions. (B) Single nucleotide substitutions in the FBEa. “x” indicates position of the substitutions; every nucleotide identity was assayed at each position. (Box) The core element. β-Galactosidase values are relative to the wild-type FBEa; error bars represent the standard deviation of six repetitions. The wild-type identity is labeled at each position. (C) Summary. A summary sequence of the FBF-1 core binding element based on the experiments in A and B.