FIGURE 7.

The 5′ intermediates are capped, whereas the 3′ intermediates are polyadenylated. Cells expressing the inducible adh reporter were codepleted of SKI2 and XRN1. In (A,B), total RNA samples were subjected to oligo(dT)-targeted RNase H cleavage. In (C,D), total RNA samples were co-immunoprecipitated with anti-cap antibodies. Northern blots were hybridized with 5′ or 3′ probes as indicated. The positions of the 3′ and 5′ intermediates are shown. In C, one-tenth of the inputs and supernatants and 100% of the immunoprecipitates were analyzed by Northern blot. In D, 100% of the inputs, supernatants, and pellets was loaded. The levels of the intermediates relative to those of the full-length transcript are indicated below the lanes.