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. 2005 Apr;11(4):470–486. doi: 10.1261/rna.7215305

FIGURE 5.

FIGURE 5.

Ribosomal dissociation activity of eIF3. Dissociation activity of eIF3j− in the presence of (A) poly(U), (C) eIF2-ternary complex (TC) and (D) poly(U), eIF1, and eIF1A. (B) Dissociation activity of eIF3j+ in the presence of poly(U), eIF1, and eIF1A. (E,F) Resistance of 80S ribosomes to dissociation by (E) eIF1 or eIF1A alone and (F) eIF3c (p110 subunit) alone, with eIF1 or with eIF1 and poly(U). The optical density of ribosomal profiles was measured after centrifugation through 10%–30% linear sucrose gradients. Sedimentation was from right to left. Upper fractions from gradients have been omitted for clarity. (G) Dissociation by eIF3j− (in the presence and absence of poly(U)) of 80S complexes assembled on [32P]-CAAn-GUS mRNA and purified by sucrose density gradient centrifugation. After incubation with eIF3j− and poly(U) as indicated, ribosomal complexes were again centrifuged through 10%–30% linear sucrose gradients. Sedimentation was from right to left. Upper fractions from the gradient have been omitted for clarity.

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