FIGURE 7.
(A–G) Stimulation of the binding of the eIF2•GTP•Met-tRNAiMet ternary complex (TC) to 40S subunits by other initiation factors and AUG triplets, as indicated. Ribosomal complexes were fractionated by centrifugation in 10%–30% linear sucrose gradients and analyzed by scintillation counting of [35S]Met-tRNAiMet. Sedimentation was from right to left. Upper fractions from each gradient have been omitted for clarity.