TABLE 3.
The predicted modifications on T. brucei rRNA compared to plants, humans, and yeast
| T. brucei | Plant | Human | Yeast | ||||||
| Site | SnoRNA | snoRNA | Site | snoRNA | Site | snoRNA | Site | Conservation of sites | |
| SSU | Cm16 | TB10Cs2C1 | T | ||||||
| SSU | Um36 | TB10Cs2C1 | T | ||||||
| SSU | Cm46 | TB8Cs3C3 | T | ||||||
| SSU | Am56 | TB8Cs2C1 | AtU27 | Am28 | U27 | Am27 | SnR74 | Am28 | PHYT |
| SSU | Cm66 | TB8Cs2C1 | AtsnoR66 | Cm38 | PT | ||||
| SSU | Am125 | TB8Cs1C2 | Am98 | SnR51 | Am100 | YHT | |||
| SSU | Um680 | TB10Cs3C3 | AtsnoR77Y | Um580 | Nd | Um627 | SnR77 | Um578 | PHYT |
| SSU | Um714 | TB9Cs2C6 | AtsnoR13 | Um613 | PT | ||||
| SSU | Am721 | TB6Cs2C1 | AtU36 | Am621 | U36a | Am668 | SnR47 | Am619 | PHYT |
| SSU | Gm1517 | TB11Cs3C2 | T | ||||||
| SSU | Gm1603 | TB8Cs3C2 | T | ||||||
| SSU | Um1652 | TB10Cs1C3 | T | ||||||
| SSU | Um1674 | TB10Cs3C2 | U33 | Um1270 | U33 | Um1326 | SnR55 | Um1267 | PHYT |
| SSU | Gm1676 | TB9Cs3C1 | AtsnoR21 | Gm1272 | U32 | Gm1328 | SnR40 | Gm1269 | PHYT |
| SSU | Cm1824 | TB3Cs2C1 | T | ||||||
| SSU | Gm1895 | TB9Cs2C4 | AtsnoR19 | Gm1431 | U25 | Gm1490 | SnR56 | Gm1427 | PHYT |
| SSU | Um1899 | TB11Cs2C1 | T | ||||||
| SSU | Gm1931 | TB10Cs4C4 | T | ||||||
| SSU | Cm1932 | TB10Cs4C2 | T | ||||||
| SSU | Um2054 | TB8Cs2C0 | T | ||||||
| SSU | Am2096 | TB9Cs3C2 | T | ||||||
| SSU | Um2123 | TB10Cs4C3 | AtU43 | Cm1641 | U43 | Cm1705 | SnR70 | Cm1638 | PHYT |
| SSU | Gm2227 | TB8Cs1C1 | AtsnoR23 | Am1754 | Nd | Am1850 | Nd | Am1779 | PHYT |
| 5.8s | Um7 | TB10Cs1C3 | T | ||||||
| 5.8s | Um32 | TB10Cs4C1 | T | ||||||
| 5.8s | Gm75 | TB8Cs3C3 | AtsnoR39BY | Gm79 | Nd | Gm75 | PHT | ||
| 5.8s | Am163 | TB9Cs4C2 | T | ||||||
| LSU5 | Am420 | TB10Cs3C2 | T | ||||||
| LSU5 | Am907 | TB9Cs2C2 | T | ||||||
| LSU5 | Am910 | TB10Cs2C2 | AtU18 | Am660 | U18 | Am1313 | U18 | Am647 | PHYT |
| LSU5 | Cm911 | TB10Cs2C2 | U18 | Cm648A | YT | ||||
| LSU5 | Gm920 | TB6Cs2C2 | T | ||||||
| LSU5 | Cm924 | TB9Cs2C2 | AtsnoR58Y | Cm674 | Nd | Cm1327 | SnR58 | Cm661 | PHYT |
| LSU5 | Um1078 | TB9Cs2C3 | T | ||||||
| LSU5 | Um1080 | TB11Cs4C2 | T | ||||||
| LSU5 | Gm1089 | TB9Cs2C3 | AtsnoR39BY | Gm812 | Nd | Gm1509 | SnR39B | Gm803 | PHYT |
| LSU5 | Am1091 | TB11Cs4C2 | AtU51 | Am814 | U51/U32a | Am1511 | SnR39/59 | Am805 | PHYT |
| LSU5 | Am1150 | TB10Cs4C5 | T | ||||||
| LSU5 | Am1160 | TB9Cs5C2 | AtsnoR72Y | Am883 | SnR72 | Am874 | PYT | ||
| LSU5 | Cm1170 | TB9Cs5C1 | T | ||||||
| LSU5 | Am1188 | TB11Cs1C3 | T | ||||||
| LSU5 | Gm1192 | TB9Cs2C5 | AtU80 | Um915 | U80 | Gm1612 | SnR60 | Gm906 | PHYT |
| LSU5 | Um1309 | TB10Cs3C4 | T | ||||||
| LSU5 | Am1344 | TB9Cs5C1 | T | ||||||
| LSU5 | Um1345 | TB10Cs3C4 | AtsnoR41Y | Um1064 | PT | ||||
| LSU5 | Cm1420 | TB3Cs2C1 | T | ||||||
| LSU5 | Gm1431 | TB9Cs4C1 | AtU38 | Am1140 | U38ab | Am1858 | SnR61 | Am1131 | PHYT |
| LSU5 | Cm1772 | TB8Cs3C1 | AtU24 | Cm1439 | U24 | Cm2338 | U24 | Cm1435 | PHYT |
| LSU5 | Am1784 | TB8Cs3C1 | AtU24 | Am1451 | U76 | Am2350 | U24 | Am1447 | PHYT |
| LSU5 | Gm1785 | TB8Cs3C1 | Gm2351 | U24 | Gm1448 | TYH | |||
| LSU5 | Gm1798 | TB8Cs1C3 | T | ||||||
| LSU5 | G1873 | TB8Cs1C1 | T | ||||||
| LSU5 | Cm1890 | TB10Cs4C5 | T | ||||||
| LSU5 | Cm2251 | TB11Cs1C1 | T | ||||||
| LSU3 | Gm11 | TB9Cs1C1 | AtU34 | Um1882 | U34 | Um2824 | SnR62 | Um1886 | PHYT |
| LSU3 | Gm319 | TB9Cs4C3 | T | ||||||
| LSU3 | Am342 | TB6Cs1C2 | T | ||||||
| LSU3 | Um360 | TB11Cs2C1 | T | ||||||
| LSU3 | Am481 | TB6Cs1C3 | SnR13 | Am2278 | YT | ||||
| LSU3 | Am482 | TB6Cs1C3 | AtU15 | Am2271 | U15 | Am3764 | SnR13 | Am2279 | PHYT |
| LSU3 | Gm489 | TB10Cs1C4 | AtU15 | Gm2278 | SnR75 | Gm2286 | PYT | ||
| LSU3 | Um515 | TB11Cs3C1 | Um3787 | HT | |||||
| LSU3 | Am525 | TB8Cs1C3 | AtsnoR44 | Am2316 | U79 | Am3809 | PHT | ||
| LSU3 | Cm538 | TB10Cs1C1 | AtsnoR44 | Cm2327 | U74 | Cm3820 | SnR64 | Cm2235 | PHYT |
| LSU3 | Am546 | TB10Cs1C1 | T | ||||||
| LSU3 | Gm596 | TB9Cs2C7 | SnR190 | Gm2394 | YT | ||||
| LSU3 | Um611 | TB11Cs4C3 | AtsnoR53 | Um2400 | PT | ||||
| LSU3 | Um622 | TB9Cs2C7 | AtsnoR37 | Um2411 | U52 | Um3904 | SnR78 | Um2414 | PHYT |
| LSU3 | Um656 | TB10Cs3C5 | AtsnoR16.1 | Um2445 | PT | ||||
| LSU3 | Um665 | TB10Cs3C5 | T | ||||||
| LSU3 | Um949 | TB6Cs1C2 | T | ||||||
| LSU3 | Gm1000 | TB11Cs2C1 | AtU31 | Gm2610 | U31 | Gm4166 | SnR67 | Gm2616 | PHYT |
| LSU3 | Um1031 | TB5Cs1C1 | AtsnoR10 | Um2641 | Nd | Um4197 | PHT | ||
| LSU3 | Cm1113 | TB5Cs1C1 | T | ||||||
| LSU3 | Gm1183 | TB11Cs1C2 | AtsnoR1 | Gm2781 | SnR48 | Gm2788 | PYT | ||
| LSU3 | Cm1202 | TB6Cs1C1 | T | ||||||
| LSU3 | Gm1207 | TB11Cs1C2 | AtsnoR38Y | Gm2805 | SnR38 | Gm2812 | PYT | ||
| LSU3 | Um1313 | TB10Cs3C1 | snR52 | Um2918 | YT | ||||
| LSU3 | Gm1314 | TB10Cs3C1 | Gm4459 | snR52 | Gm2919 | TYH | |||
| LSU3 | Am1326 | TB11Cs4C1 | AtsnoR18 | Am2924 | PT | ||||
| LSU3 | Am1338 | TB11Cs4C1 | AtU29 | Am2936 | U29 | Am4493 | SnR71 | Am2943 | PHYT |
| LSU3 | Cm1351 | TB9Cs2C1 | AtU35 | Cm2949 | U35 | Cm4506 | SnR73 | Cm2956 | PHYT |
| LSU3 | Um1373 | TB9Cs3C3 | T | ||||||
| LSU3 | Cm1754 | TB11Cs3C1 | T | ||||||
| SSU | Ψ40 | TB10Cs1H3 | T | ||||||
| SSU | Ψ61 | TB11Cs4H2 | T | ||||||
| SSU | Ψ263 | TB10Cs3H1 | ACA10 | Ψ 214 | HT | ||||
| SSU | Ψ505 | TB10Cs4H4 | T | ||||||
| SSU | Ψ662 | TB6Cs1H3 | ACA24 | Ψ 613 | HT | ||||
| SSU | Ψ1001 | TB6Cs2C1 | T | ||||||
| SSU | Ψ1088 | TB9Cs1H1 | T | ||||||
| SSU | Ψ1113 | TB8Cs2H1 | T | ||||||
| SSU | Ψ1186 | TB10Cs4H2 | T | ||||||
| SSU | Ψ1423 | TB8Cs3H1 | T | ||||||
| SSU | Ψ1619 | TB9Cs1H2 | T | ||||||
| SSU | Ψ2123 | TB9Cs4H1 | T | ||||||
| LSU5 | Ψ824 | TB6Cs1H4 | T | ||||||
| LSU5 | Ψ901 | TB10Cs1H2 | T | ||||||
| LSU5 | Ψ1103 | TB9Cs3H2 | AtsnoR77 | 826 | Nd | 1523 | PHT | ||
| LSU5 | Ψ1250 | TB9Cs1H3 | T | ||||||
| LSU5 | Ψ1412 | TB9Cs2H2 | T | ||||||
| LSU5 | Ψ1773 | TB10Cs4H3 | T | ||||||
| LSU5 | Ψ2248 | TB10Cs4H1 | T | ||||||
| LSU3 | Ψ360 | TB9Cs4H4 | T | ||||||
| LSU3 | Ψ380 | TB6Cs1H1 | T | ||||||
| LSU3 | Ψ397 | TB10Cs3H2 | SnR32 | 2191 | YT | ||||
| LSU3 | Ψ475 | TB11Cs3H2 | T | ||||||
| LSU3 | Ψ566 | TB11Cs4H3 | T | ||||||
| LSU3 | Ψ617 | TB9Cs2H1 | T | ||||||
| LSU3 | Ψ659 | TB10Cs1H1 | T | ||||||
| LSU3 | Ψ1167 | TB10Cs2H1 | AtsnoR93 | 2773 | Nd | 4330 | PHT | ||
| LSU3 | Ψ1208 | TB9Cs3H1 | AtU65 | 2816 | U65 | 4373 | SnR34 | 2823 | PHYT |
| LSU3 | Ψ1272 | TB9Cs1H1 | AtU65 | 2870 | U65 | 4427 | SnR34 | 2877 | PHYT |
| LSU3 | Ψ1308 | TB11Cs3H1 | T | ||||||
| LSU3 | Ψ1336 | TB9Cs2H2 | ACA10 | Ψ 4492 | SnR37 | 2941 | HTY | ||
| LSU3 | Ψ1357 | TB11Cs4H1 | T |
The predicted modifications in T. brucei (T) are compared with their counterparts in S. cerevisae (Y), plants (Arabidopsis) (P), and humans (H). The target site on the respective rRNA is given. Nm and Ψ designate 2′-O-methylation and pseudouridine, respectively. The modifications shared by other species are indicated. There are collectively 84 Nms; 44 sites are conserved with at least one other organism; 36 are conserved between trypanosomes and plants; 34 between trypanosomes and yeast; and 30 between trypanosomes and humans. Among the 32 predicted pseudouridines, only nine are conserved.