FIGURE 1.

61 mer MAPK14 dsRNA containing blunt and overhang ends are processed in distinctly different manners. (A) Dicer cleavage of [³²P]-5′ sense labeled dsRNA with two blunt ends (blunt–blunt) and two 2-nt overhang ends (over–over) analyzed by denaturing PAGE. Incubation times in minutes. RNase T1 digest (T1) and hydroxyl digest (OH) are labeled at the tops of the lanes for both substrates. The primary and secondary cleavage product bands that result from Dicer entering from the 5′ sense (left) and 3′ sense (right) ends are indicated in red and black, respectively. (B) Diagrams of Dicer processing of blunt and/or overhang ended MAPK14 61mer dsRNA substrates. Red and black arrows show Dicer positions of cleavage from the 5′ sense (left) and 3′ sense (right) ends, respectively. Solid arrows were derived from Dicer digestion of [³²P]-5′ sense or antisense labeled dsRNA. Length of arrows indicates intensity of cleavage. Processing positions with dashed arrows were derived from processing of blunt secondary products (Supplementary Fig. 1 at www.dharmacon.com/tech/publications).