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siRNA activity is affected by asymmetric end structure. Silencing efficiency of siRNAs containing blunt and/or overhang ends at different concentrations (over–over [white], over–blunt [red], blunt–over [cyan], and blunt–blunt [blue] for siRNAs 1, 2, 3, and 4 in A,B,C,D, respectively). Silencing is expressed as a ratio of Cyclophilin B and house keeping gene (HKG) mRNA expression.
