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. 2005 May;11(5):728–738. doi: 10.1261/rna.7134305

FIGURE 2.

FIGURE 2.

Analysis of the growth phenotypes conferred by the L20Δ1, L20Δ2, and L20ΔN truncations. (A,B) L20Δ1 and L20Δ2 display complementation defects. The E. coli ΔrplT strain IBPC6801 was transformed with either plasmid pBL20ec, expressing wild-type L20 (L20 sector) or its derivatives pBL20ecΔ6–20 (L20Δ1 sector) or pBL20ecΔ6–29 (L20Δ2 sector) expressing the L20Δ1 and L20Δ2 truncations, respectively. The pBL20ecΔ6–20 and pBL20ecΔ6–29 transformants were cotransformed with the compatible plasmids pSGL6 expressing wild-type L20 (L20Δ1 + L20 and L20Δ2 + L20 sectors) or pSUrplTW61Stop expressing the N-terminal domain of L20 (L20Δ1 + L20N and L20Δ2 + L20N sectors). Growth was followed at 37°C upon induction of the expression of the different rplT alleles with 1 mM IPTG (A) and in noninducing conditions (B). (C) Overproduction of L20ΔN results in a dominant-lethal phenotype. The E. coli IBPC5311[λMQ21ΔNB] lysogen was transformed with either pBR322 (empty vector sector) or with plasmids pBL20ec (L20 sector) or its derivative pBL20ecΔN expressing the L20ΔN truncation (L20ΔN sector). The pBL20ecΔN transformant was cotransformed with the compatible plasmids pSGL6 expressing wild-type L20 (L20ΔN + L20 sector) or pSUrplTW61Stop expressing the N-terminal domain of L20 (L20ΔN + L20N sector). Induction was carried out with 1 mM IPTG.