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. 2005 May;11(5):728–738. doi: 10.1261/rna.7134305

FIGURE 3.

FIGURE 3.

Accumulation of wild-type L20 and of L20Δ1 and L20Δ2 truncations. Total extracts from uninduced (lanes 1,3,5) and induced (lanes 2,4,6) E. coli strain IBPC6801 transformed either with plasmid pBL20ec, expressing wild-type L20 (lanes 1,2) or with plasmids pBL20ecΔ6–20 (lanes 3,4) and pBL20ecΔ6–29 (lanes 5,6), expressing the L20Δ1 and L20Δ2 truncations, respectively, were fractionated on a 17.5% SDS-PAGE gel and subjected to Western blotting analysis using polyclonal antibodies raised against the L20ΔN truncation and PTH protein. L20 and L20ΔN truncation (lane 7) were used as size markers.