FIGURE 3.

(A–H) Northern hybridizations with comprehensive sets of labeled oligonucleotides covering the sense DNA strand. (A) rnl, rns; (B) rnpB; (C) atp6, atp8, atp9; (D) rps3; (E) cox1, cox2, cox3; (F) cox1I1b/I2b; (G,H) cobE1/E2/I1. In cells grown under standard conditions (3% glucose), only small concentrations of RNA-processing intermediates are present (E–H). The most prominent type of RNA intermediates represent pre-mRNAs and splicing intermediates of the mosaic genes cox1 (E,F) and cob (G,H). Mitochondrial RNA prepared from an intron-less strain (P3) was used to identify mRNA (A–H, first lanes), and the mutant R10/5 defective in cobI1 splicing was used to identify cobI1 precursors (G,H, second lanes).