FIGURE 6.

Various alternatives of fusion proteins produced by slippage by different tRNAs. After PreScission Protease digestion the GST part is cleaved away and the molecular mass of the resulting peptides is analyzed. (I) The peptide part liberated after the cleavage if no frame-shifting occurred; the coding sequence is also presented. The critical AGA codon for R (arginine) is indicated. (II) Fragments of different peptides liberated after the cleavage if different tRNAs were slipping. The amino acid inserted by the frameshifting tRNA and the sequence where this tRNA frameshifts are in bold. The sequence translated and amino acids inserted in the +1 frame are in italic. On the right side are the expected masses of those hypothetical peptides.