FIGURE 1.

Growth defect of trm8-Δ/trm8-Δ and trm82-Δ/trm82-Δ strains on glycerol-containing minimal media. Homozygous diploid trm8-Δ/trm8-Δ, trm82-Δ/trm82-Δ, or wild-type strains, containing a CEN URA3 plasmid bearing TRM8 or TRM82 under control of its own promoter as indicated, were grown overnight, and 10-fold serial dilutions were plated on synthetic media lacking uracil. (A) Cells plated on glucose-containing media, incubated for 2 d at 30°C. (B) Cells plated on glycerol-containing media, incubated for 7 d at 38°C.