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Trm8p cross-links to pre-tRNAPhe in the presence and absence of Trm82p. Preparations of yeast Trm82p, Trm8p, Trm8p/ Trm82p, or similarly purified control protein 3C protease were subjected to UV cross-linking with pre-tRNAPhe as described in Materials and Methods, followed by RNase treatment and resolution by 10% SDS-PAGE. (A) Analysis of proteins after SDS-PAGE and Coomassie staining. (B) Analysis of radioactivity incorporated in gel shown in A.
