Table 2. Round 9 clones.

	Insertion position	Inserted sequencea (5′→3′)	Mutations in the constant regionb	Activityc (kobs, per h)
1	P7.1	GGCACCAGUAAGUGUGGAUUGUACAUGAAUUUCCAAAAAUAACUUCGAGCCC	A896G (L7.2)	0.58
2	P9	GGCACCAAGCGAGAUGUAAUUUUUCGUUACUCUGGGUGUGGACAGGAGCCC	None	5.94
3	P7.1	GGCACCAGCUCUAUGAGAGUGGCCUUACGGAUGUGGAUAGAUAACCGGGAGCCC	None	0.68
4	P8	GGCACCAGAAGUAAUUUCUAUGAUUUUGGCGUUUAUUUCGAUGGAUGGAGCCC	A897G (L7.2)	3.72 (1.69)d
5	P8	GGCACCUAUAAGUUCCAAAAACUCUUUUCCUUCUCUGAUGUGGUUGAGCCC	ΔA977 (L9.1)	0.29
6	P9	GGCACCGCCCUUGAGAAAUUUGCUAGGGUCGUGUGAUGUGGUCACGAGCCC	None	1.34
7	P9	GGCACCCGAUAAGAGGUAGCAUAGUCGAUGUGUGGAAGUGAUAUCAAGAGCCC	A928G (L8)	2.16
8	P7.1	GGCACCGACUCCGUUCGGAGCGGUUCUGCGUGGAGAUGACAUUUAGGAGCCC	ΔG958 (L9)	1.05
9	P7.1	GGCACCAUUUCGAUAGGUAAGGGAUGACCCUGUGGGUAGCAUUUGCGAGCCC	U34C (J2/3) ΔG891 (P7.2)	1.52
10	P7.1	GGCACCAAACGAUAUCGUACGUGGAAAUGUUGCUCCUGAUAUACAAUGAGCCC	A918G (P8)	0.67
M1 mutant				6.35 × 10–3

^aThe restriction sites are shown in italic. Bold letters indicate the sequences complementary to the 5′ leader of the substrate RNA.

^bNumbering of the sequence is according to Belfort et al. (36).

^cActivity (k_obs) was determined in the presence of 5 µM substrate RNA.

^dThe activity of the clone 4 derivative which lacks the point mutation in the constant region.