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| T4 td ribozyme variants | kcata (per h) | Km (µM) | kcat/Km (per h/µM) |
|---|---|---|---|
| Wild-type | 24.61 | 5.53 | 4.45 |
| M1 mutant | NAb | NAb | 1.27 × 10–3 |
| Clone 2 | 6.44 | 1.29 | 4.99 |
| Clone 2.1 | 5.01 | 0.40 | 12.50 |
aIt was not determined whether kcat values reflect the rate of the chemical step.
bNot available. Because of its high Km value (>50 µM), kcat and Km values of the M1 mutant were undetermined.
