Table 4. Round 11 clones.
Clone | Inserted sequencea (5′→3′) | Activityb |
---|---|---|
2 | GGCACCAAGCGAGAUGUAAUUUUUCGUUACUCUGGGUGUGGACAGGAGCCC | 1.0 |
2.1 | GGCAAUGAGCGAGAUGUAAUUUUUCGUUUAUCUGGGUGUGGACAGGUGCC | 2.5 |
2.2 | GGCAGUGAGCGAGAUGUAAUUUUUCGUUCAUCUGGGUGUGGACAGGUUGCC | 2.3 |
2.3 | GGCUCUGAGCGAGAUGUAAUUUUUCGUUUCUCUGGGUGUGGACAGGUGCC | 1.4 |
2.4 | GGCAAGAAGCGAGAUGUAAUUUUUCGUUCGUCUGGGUGUGGACAGGUAGCC | 1.3 |
aThe randomized positions are shown in bold. Underlined letters indicate the sequences that coincide with the consensus sequence.
bThe activity relative to the clone 2 ribozyme was determined with 0.5 µM substrate RNA.