TABLE 1.

Values obtained from the time-resolved FRET data for the RNA-L7Ae complex, and the fitting statistics

Model	Parametera	Valueb	95% C.I.c	χ2
1 Gaussiand	r	40.02	39–41.1	11.7
	fwhm	25.2	24.1–26.3
2 Gaussian globale	r1	43.4	42.1–44.1	10.9
	fwhm1	9.8	2.9–16.9
	r2	70.6	69.5–71.5
	fwhm2	11.6	Fixed
	p	0.149	0.072–0.205
2 Gaussianf	r1	43.4	42.0–44.2	10.7
	fwhm1	8.8	0.0–16.9
	r2	69.6	Fixed
	fwhm2	14.6	Fixed
	p	0.169	0.079–0.221

^ar, r1 and r2 are donor-acceptor (D-A) distances and fwhm are full widths at half maxima. p-values are the amplitudes of the long distance, relative to a short distance amplitude of unity.

^bAll distances are given in Å.

^c95% confidence interval.

^dResult of fitting the data for the RNA in the presence of L7Ae to a single Gaussian function of D-A distances.

^eResult of fitting the data for the RNA both in the presence and absence of L7Ae globally to two Gaussian functions of D-A distances. The results are only shown for the RNA in the presence of L7Ae. The halfwidth of the longer distance was fixed for this calculation.

^fResult of fitting the data for the RNA in the presence of L7Ae to two Gaussian functions of D-A distances. The longer distance and halfwidth were fixed for this calculation.