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. 2005 Aug;11(8):1225–1237. doi: 10.1261/rna.2760705

FIGURE 2.

FIGURE 2.

Methionine 368 of the Rnt1p dsRBD is mildly required for processing of a subset of Rnt1p substrates in vivo. (A) Northern blot analysis of the processing of known Rnt1p substrates in a wild-type strain (lane 1), a strain lacking Rnt1p (lane 2), and strains expressing the K371A, M368A, and M368E dsRBD mutant versions of Rnt1p (lanes 3, 4, and 5, respectively). M, mature snoRNAs; U, unprocessed precursors. (B) Predicted secondary structure of the stem-loop recognized by Rnt1p on the precursor of snR36 snoRNA. Arrowheads indicate the cleavage site (Chanfreau et al. 1998a).