Figure 5.

Ω and a 5′-cap stimulate first-round translation. Translation from capped and uncapped Ω-luc-A₅₀ (A) or luc-A₅₀ (B) mRNAs was examined immediately following their delivery to carrot cells. The translation from capped, poly(A)^– or poly(A)⁺ luc mRNAs (C) or from luc mRNA terminating in the TMV 3′-UTR (D) was also determined. The resulting luciferase activity was plotted as a function of time. The fold increase in expression conferred by Ω (A), 5′-cap (B), poly(A) tail (C) or TMV 3′-UTR (D) relative to the control mRNA at each time point analyzed is indicated as the expression ratio below each graph.