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Depletion of eIF4F, eIFiso4F and PABP from wheat germ lysate. Wheat germ lysate was incubated with (A) m7GTP-Sepharose or (B) poly(A)-Sepharose for 30 min. Western analysis was performed to determine the level of eIF4G, eIF4E, eIFiso4G, eIFiso4E, eIF4A, eIF4B, PABP and Hsp101 in depleted lysate relative to unfractionated lysate.
