Figure 9.

eIF4F but not eIFiso4F mediates Ω function in uncapped mRNAs. PABP-depleted, eIF4F/eIFiso4F-reduced wheat germ lysate was programmed with uncapped luc-A₅₀ mRNA (A) or uncapped Ω-luc-A₅₀ (B) and was supplemented with the indicated amounts of eIF4F or eIFiso4F (each including eIF4A). mRNA constructs were translated in triplicate and the average value and standard deviation for each construct is reported. Luciferase expression is indicated as light units from 2 µl of translation lysate. The degree to which Ω stimulated translation relative to the control construct is indicated in (C).